Detector with a fixed wavelength, managed by a specific lamp.To be detected a compounds must have a chromophore in its molecular structure (i.e substances with aromatic ring, with at least 2 conjugated double bonds, with a double bond adjacent to an atom with ion electron pairs, with carbonyl groups, or containing bromine, iodine, or sulfur).ĭifferent UV detectors are commonly used: The UV detectors are not universal detectors. The signal collected correspond to a light absorption. When subjected to a light radiation, certain functional groups may be the seat of electronic excitation corresponding to an energy absorption at a specific wavelength. UV Visible Detector – Diode Array (DAD) Technology To maximize the dectection potential it is recommanded to couple different devices ie. Detection sensitivity is different from a detector to another, and is linked to compounds concentration or not. Collect can be done relatively to a threshold and/or a slope value. The collected fractions are cutted based on the dectector signal. Detectors distinguish compounds from the mobile phase according to their physical properties.
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